Proteomics Core Facility Services (LaP)

an ideal tool to study cellular topography

The Proteomics Core Facility Services (Lap), at University of Bologna provides services to which you may send your samples for analysis. These services include analytical and preparative high-resolution !D and 2-D PAGE (including spot analysis and cutting), automated two-dimensional HPLC protein fractionation system (PF2D), multiplex suspension array technology system (Bioplex 200) and, in collaboration with other centers, mass spectrometry analysis for protein identification and mass determination.

Analyses

Sample preparation and protein extraction

Protein Separation by 1-DE
* Native and denaturing gels
* Multiple protein detection with ECL plex fluorescent Western Blotting
* PVDF Fluorescence Analyses

Protein Separation by 2-DE
* Minigels 2-DE and Silver staining (8 x 9 cm)
* Minigels 2-DE and Coomassie/Fluorescence staining
* Minigels 2-DE and PVDF transfert

* Maxi 2-DE and Silver staining (18 x 20 cm)
* Maxi 2-DE and Coomassie/Fluorescence staining
* Maxi 2-DE and PVDF transfert

Protein Separation by PF2D (Beckman)
*First dimension HPCF column
*Second dimension HPRP column

Multiplex analysis system (Luminex 200)
*signal transduction molecules
*cytokines
*other proteins/peptides

Protein identification (from 1-DE, 2-DE gels and liquids)
* Maldi Tof
* NanoLC - ESI-MS/MS*cytokines
* Shotgun Proteomics and Biomarker Discovery

Protein and peptide mass determination

Data analysis and training
* Data analysis
* Training Courses

 

Contact

Dr. Francesca Borsetti

francesca.borsetti@unibo.it

Dr. Enzo Spisni

enzo.spisni@unibo.it

Tel. +39051.209.4133

Confocal Microscopy Lab