Confocal Microscopy Lab (CLSM)
The confocal microscope is a tool allowing researchers to examine ocular tissues in a specific way and thus opening the door to new investigation and collaborations. The microscope has a shallow depth field so an image is collected from an optical diction rather than from the full depth of the specimen as in conventional light microscopy.
Another advantage of confocal microscopy is that specimens can be optically sectioned not only in the XY plane but also vertically in the Z plane. Images can be viewed in three dimensions, and can even be rotated in space to obtain views of the specimen from all directions. Furthermore, the CLMS has better transverse resolution than conventional optical microscopes, and can measure height difference up to 170 µm.
Applications
The 3D-imaging properties of the CLSM make it an ideal tool to study cellular topography, internal structure, and fluorescence properties of biological objects and materials with dimensions in the sub-mm to mm range.
The available equipment includes:
- Confocal microscope Leica, model TCS SP2
- Krypton-argon laser beam
- Infrared laser beam
If you are interested in more information please contact:
Prof. Carlo Taddei
e-mail: carlo.taddei@unibo.it
