Integrated Live Cell Imaging Lab
The integrated “Live-Cell Imaging” system is composed of:
- an inverted epifluorescence microscopy Nikon Diaphot 300, with 10x,
40x and 61x objectives;
- a back-illuminated CCD camera (Photometrics Cascade, Princeton Instr.,
Trenton, NJ);
- a motorized wheel (Sutter) for different excitation wavelenghts
acquisition/analysis software Metamorph/Metafluor (Universal Imaging,
Downingtown, PA, USA), with algorithms for image deconvolution and 3D
recontruction.
The microscope is equipped with filter sets for fluorescein, rhodamine, fluo-3, DAPI, and most common GFPs (GFP, YFP, CFP). The motorized multifilter wheel allows ratiometric measurements at excitation wavelenghts or simultaneous determination of three fluorescent probes.
The digital camera is characterized by very high acquisition rate (5-10 MHz), which can be further increased by reducing the areas of interest o image binning.
Prof. Michela Rugolo is in charge of the imaging system, taking advantage of the excellent expertise of Dr. Anna Maria Porcelli and dr. Anna Ghelli.
