Approfondimento sulla specificità del legame peptidico al C-terminale del dominio PDZ dell’ossido nitrico sintasi neuronale

Studi precedenti hanno dimostrato che il c-jun e l’ossido nitrico sintasi neurale (nNOS) sono indotti nei motoneuroni danneggiati, ma il loro ruolo nella morte dei motoneuroni rimane poco chiaro.

  • Abbiamo ipotizzato che nNOS potrebbe essere un ulteriore effettore della chinasi N-terminale c-jun (JNK) / c-jun nella morte per distacco del motoneurone. Qui abbiamo scoperto che il distacco della radice brachiale ha indotto un aumento transitorio dell’attività di JNK e un aumento di tre e quattro volte rispettivamente di fosfo-c-jun e c-jun; tuttavia, il distacco brachiale ha ridotto l’espressione della proteina nNOS da 4 ore a 14 giorni dopo la lesione.
  • A 14 giorni dalla lesione, quasi tutti i motoneuroni nNOS-positivi erano localizzati insieme ai motoneuroni fosfo-c-jun positivi nelle corna addominali omolaterali.
  • L’inibitore JNK SP600125, applicato immediatamente dopo la lesione, ha aumentato il livello della proteina nNOS sia nel midollo spinale che nei motoneuroni feriti e ha causato una leggera riduzione della morte dei motoneuroni inibendo la fosforilazione di c-jun 14 giorni dopo la lesione.
  • I nostri risultati hanno mostrato che la via di trasduzione del segnale JNK / c-jun è coinvolta nel distacco della radice. L’inibizione della fosforilazione di c-jun impedisce che i livelli di nNOS scendano al di sotto della linea di base nel midollo spinale e mitiga parzialmente la morte del motoneurone dopo l’estrazione della radice.
  • Pertanto, l’inibizione della fosforilazione di c-jun o l’aumento del livello della proteina nNOS in un midollo spinale danneggiato in una fase iniziale potrebbero essere utilizzati in futuro come strategie di bersaglio molecolare per prevenire la degenerazione dei motoneuroni durante l’eliminazione delle radici.

 

La coda C-terminale (CT) dell’ossido nitrico sintasi neuronale (nNOS) è un elemento regolatorio che inibisce l’attività di nNOS in assenza di calmodulina legata (CaM).

 

Struttura cristallina del dominio nNOS reduttasi (nNOSr) (Garcin, ED, Bruns, CM, Lloyd, SJ, Hosfield, DJ, Tiso, M., Gachhui, R., Stuehr, DJ, Tainer, JA e Getzoff, ED (2004 ) J Biol. Chem. 279, 37918-37927) ha descritto come la prima metà di CT interagisce con nNOSr e ha quindi fornito un modello per uno studio dettagliato. Abbiamo generato mutanti troncati in nNOS e nNOSr per testare l’importanza di 3 diverse regioni CT.

  • L’eliminazione della metà terminale di CT (tutti i residui da Ile1413 a Ser1429), che è invisibile nella struttura cristallina, non ha avuto quasi alcun effetto sul rilascio di NADP +, riduzione della flavina, autoossidazione della flavina, riduzione dell’eme, attività reduttasi o NO attività di sintesi, ma ha impedito un aumento della schermatura FMN che normalmente si verifica in risposta al legame NADPH.
  • Un’ulteriore rimozione  dell’alfa-elica CT (residui da 1401 a 1412) ha aumentato significativamente il tasso di rilascio di NADP +, l’autoossidazione della flavina e l’attività della NADPH ossidasi e ha provocato un’esposizione eccessiva del cofattore FMN. Questi effetti sono stati associati con una maggiore attività della reduttasi e una riduzione leggermente ridotta dell’eme e della sintesi di NO.
  • L’ulteriore rimozione dei residui dopo Gly1396 (troncamento CT completo) ha migliorato gli effetti sopra menzionati e ha ulteriormente modificato l’interazione di NADP + con FAD, attenuando la soppressione cinetica della riduzione della flavina e ulteriormente ridotta la riduzione dell’eme e la sintesi di NO.
  •  I nostri risultati mostrano che la TC esercita effetti sia sfaccettati che regiospecifici sull’attività catalitica e sui comportamenti correlati, fornendo così nuove informazioni sui meccanismi che regolano la catalisi di nNOS.

I domini PDZ sono motivi di interazione proteica multifunzionale che spesso si legano al C-terminale delle proteine ​​​​bersaglio.

 L’ossido nitrico (NO), una molecola di segnalazione endogena, svolge un ruolo chiave nel funzionamento del sistema nervoso, immunitario e cardiovascolare. Sebbene ci siano molte funzioni fisiologiche per l’NO neuronale prodotto principalmente dalla NO sintasi neuronale (nNOS), l’eccessiva attività di nNOS media il danno cerebrale nell’ischemia cerebrale e nei modelli animali del morbo di Parkinson.

Pertanto, la localizzazione subcellulare dell’attività di nNOS deve essere strettamente regolata. Per determinare i ligandi del dominio nNOS PDZ, abbiamo cercato 13 miliardi di peptidi diversi e abbiamo scoperto che il dominio nNOS-PDZ si lega strettamente ai peptidi che terminano in Asp-X-ValQuesto differisce dall’unico consenso noto (Thr / Ser) -X-Val che interagisce con i domini PDZ di PSD-95. La preferenza per Asp in posizione -2 è mediata da Tyr-77 di nNOS. La sostituzione di Y77D78 con H77E78 cambia la specificità del legame da Asp-X-Val a Thr-X-Val. A seguito del consenso di Asp-X-Val, sono state identificate le proteine ​​candidate che interagiscono con nNOS, inclusi i recettori del glutammato e della melatonina. I nostri risultati mostrano che i domini PDZ hanno una chiara specificità di legame peptidico.

 

L’ossido nitrico sintasi neurale, a differenza delle sue controparti endoteliali e inducibili, mostra un dominio PDZ (PSD-95 / Dlg / ZO-1) situato al suo N-terminale coinvolto nel targeting subcellulare.

 Il C-terminale delle varie proteine ​​cellulari si inserisce nel solco di legame di questo dominio PDZ e definisce la distribuzione subcellulare della NOS neuronale (nNOS). I meccanismi molecolari alla base di queste interazioni sono poco conosciuti poiché il dominio nNOS PDZ può sembrare mostrare specificità di legame di classe I, classe II e classe III.

Inoltre  , è stato recentemente suggerito che il dominio nNOS PDZ si leghi con un’affinità molto bassa al C-terminale delle proteine ​​bersaglio e che la necessaria interazione laterale concomitante debba avvenire affinché avvenga il legame. Descriviamo qui che un dominio nNOS PDZ può comportarsi come un dominio PDZ di classe III in buona fede e legarsi a sequenze C-terminali con residui acidi nella posizione P-2 con costanti micromolari basse. Può verificarsi anche il legame alle sequenze C-terminali con un residuo idrofobo in P-2 e un residuo acido in P-3 (classe II), sebbene interazioni che coinvolgono residui che si estendono a P-7 mediano questo tipo di legame.

Questo comportamento promiscuo si estende anche alla sua associazione con sequenze di classe I, che devono mostrare un residuo di Glu in P-3 e un residuo di Thr in P-2. Mediante mutagenesi sito-diretta e spettroscopia NMR, siamo stati in grado di identificare i residui coinvolti in ogni tipo specifico di legame e razionalizzare i meccanismi utilizzati per riconoscere i partner vincolanti.

 

Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E03C2363-192T BlueGene 192 tests 1524 EUR

Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E03C2363-48 BlueGene 1 plate of 48 wells 624 EUR

Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E03C2363-96 BlueGene 1 plate of 96 wells 822 EUR

Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E01C2363-192T BlueGene 192 tests 1524 EUR

Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E01C2363-48 BlueGene 1 plate of 48 wells 624 EUR

Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E01C2363-96 BlueGene 1 plate of 96 wells 822 EUR

Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E09C2363-192T BlueGene 192 tests 1524 EUR

Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E09C2363-48 BlueGene 1 plate of 48 wells 624 EUR

Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E09C2363-96 BlueGene 1 plate of 96 wells 822 EUR

nNOS (Neuronal Nitric Oxide Synthase)

RA19011 Neuromics 100 uL 451.2 EUR

Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E05C2363-192T BlueGene 192 tests 1524 EUR

Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E05C2363-48 BlueGene 1 plate of 48 wells 624 EUR

Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit

E05C2363-96 BlueGene 1 plate of 96 wells 822 EUR

Neuronal Nitric Oxide Synthase (Nnos) Antibody

20-abx114082 Abbexa
  • 878.40 EUR
  • 477.60 EUR
  • 150 ul
  • 50 ul

nNOS-C-terminus (Neuronal Nitric Oxide Synthase)

P24287 Neuromics 50 ug Blocking Peptide 208.8 EUR

nNOS-C-terminus (Neuronal Nitric Oxide Synthase)

RA24287 Neuromics 100 uL 646.8 EUR

Rat neuronal nitric oxide synthase,nNOS ELISA Kit

YLA0034RA-48T Shanghai YL Biotech 48T 465 EUR

Rat neuronal nitric oxide synthase,nNOS ELISA Kit

YLA0034RA-96T Shanghai YL Biotech 96T 600 EUR

Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit

1-CSB-E13872h Cusabio
  • 843.60 EUR
  • 5811.60 EUR
  • 3084.00 EUR
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit

CSB-E13872h-24T Cusabio 1 plate of 24 wells 198 EUR

Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit

GA-E0550RT-48T GenAsia Biotech 48T 380.4 EUR

Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit

GA-E0550RT-96T GenAsia Biotech 96T 595.2 EUR

Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit

QY-E11102 Qayee Biotechnology 96T 433.2 EUR

Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit

CSB-E14034r-24T Cusabio 1 plate of 24 wells 198 EUR

Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit

1-CSB-E14034r Cusabio
  • 843.60 EUR
  • 5811.60 EUR
  • 3084.00 EUR
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Mouse neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit

QY-E21072 Qayee Biotechnology 96T 433.2 EUR

Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit

CSB-E13273m-24T Cusabio 1 plate of 24 wells 198 EUR

Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit

1-CSB-E13273m Cusabio
  • 843.60 EUR
  • 5811.60 EUR
  • 3084.00 EUR
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each

Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody

20-abx329607 Abbexa
  • 376.80 EUR
  • 292.80 EUR
  • 100 ug
  • 50 ug

Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit

YLA0003CH-48T Shanghai YL Biotech 48T 540 EUR

Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit

YLA0003CH-96T Shanghai YL Biotech 96T 645 EUR

ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)

KTE100825-48T Abbkine 48T 424.8 EUR

ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)

KTE100825-5platesof96wells Abbkine 5 plates of 96 wells 2702.4 EUR

ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)

KTE100825-96T Abbkine 96T 686.4 EUR

Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx595427-96tests Abbexa 96 tests 764.4 EUR

Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (HRP)

20-abx314989 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit

abx195276-96tests Abbexa 96 tests 990 EUR

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit

20-abx492066 Abbexa
  • 10282.80 EUR
  • 5472.00 EUR
  • 1262.40 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (FITC)

20-abx314990 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx256109-96tests Abbexa 96 tests 801.6 EUR

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

20-abx155900 Abbexa
  • 8684.40 EUR
  • 4626.00 EUR
  • 1074.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx055223-96tests Abbexa 96 tests 801.6 EUR

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx570103-96tests Abbexa 96 tests 801.6 EUR

Pig Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx361104-96tests Abbexa 96 tests 990 EUR

Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit

ER0144 FN Test 96T 571.5 EUR

Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit

abx195274-96tests Abbexa 96 tests 990 EUR

Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit

abx195275-96tests Abbexa 96 tests 990 EUR

Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (Biotin)

20-abx314991 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

20-abx152535 Abbexa
  • 7970.40 EUR
  • 4250.40 EUR
  • 990.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx253515-96tests Abbexa 96 tests 801.6 EUR

Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx254292-96tests Abbexa 96 tests 801.6 EUR

Sheep Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx364404-96tests Abbexa 96 tests 1111.2 EUR

Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx055268-96tests Abbexa 96 tests 801.6 EUR

Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx513552-96tests Abbexa 96 tests 801.6 EUR

Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx570102-96tests Abbexa 96 tests 801.6 EUR

Mouse NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit

EM1235 FN Test 96T 571.5 EUR

Rabbit Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx362730-96tests Abbexa 96 tests 990 EUR

Monkey Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx358861-96tests Abbexa 96 tests 990 EUR

CLIA kit for Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-CL-R0746 Elabscience Biotech 1 plate of 96 wells 700.8 EUR

Chicken Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx356455-96tests Abbexa 96 tests 990 EUR

ELISA kit for Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-EL-R1438 Elabscience Biotech 1 plate of 96 wells 640.8 EUR

CLIA kit for Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-CL-M0697 Elabscience Biotech 1 plate of 96 wells 700.8 EUR

CLIA kit for Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-CL-H0537 Elabscience Biotech 1 plate of 96 wells 700.8 EUR

ELISA kit for Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-EL-M1281 Elabscience Biotech 1 plate of 96 wells 640.8 EUR

ELISA kit for Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)

E-EL-H0742 Elabscience Biotech 1 plate of 96 wells 640.8 EUR

NOS1/nNOS ELISA Kit| Mouse Nitric Oxide Synthase 1, Neuronal ELI

EF013784 Lifescience Market 96 Tests 826.8 EUR

NOS1/nNOS ELISA Kit| Rat Nitric Oxide Synthase 1, Neuronal ELISA

EF017083 Lifescience Market 96 Tests 826.8 EUR

Guinea pig Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit

abx357551-96tests Abbexa 96 tests 990 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-192T BlueGene 192 tests 1524 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Pig Neuronal Nitric Oxide Synthase ELISA kit

E07N0069-192T BlueGene 192 tests 1524 EUR

Pig Neuronal Nitric Oxide Synthase ELISA kit

E07N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Pig Neuronal Nitric Oxide Synthase ELISA kit

E07N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-192T BlueGene 192 tests 1524 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-192T BlueGene 192 tests 1524 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Mouse Neuronal Nitric Oxide Synthase ELISA kit

E03N0069-192T BlueGene 192 tests 1524 EUR

Mouse Neuronal Nitric Oxide Synthase ELISA kit

E03N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Mouse Neuronal Nitric Oxide Synthase ELISA kit

E03N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-192T BlueGene 192 tests 1524 EUR

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Rabbit Neuronal Nitric Oxide Synthase ELISA kit

E04N0069-192T BlueGene 192 tests 1524 EUR

Rabbit Neuronal Nitric Oxide Synthase ELISA kit

E04N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Rabbit Neuronal Nitric Oxide Synthase ELISA kit

E04N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Monkey Neuronal Nitric Oxide Synthase ELISA kit

E09N0069-192T BlueGene 192 tests 1524 EUR

Monkey Neuronal Nitric Oxide Synthase ELISA kit

E09N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Monkey Neuronal Nitric Oxide Synthase ELISA kit

E09N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Guinea pig Neuronal Nitric Oxide Synthase ELISA kit

E05N0069-192T BlueGene 192 tests 1524 EUR

Guinea pig Neuronal Nitric Oxide Synthase ELISA kit

E05N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Guinea pig Neuronal Nitric Oxide Synthase ELISA kit

E05N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103472 Abbexa
  • 477.60 EUR
  • 159.60 EUR
  • 1328.40 EUR
  • 644.40 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103473 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1362.00 EUR
  • 661.20 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103474 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1362.00 EUR
  • 661.20 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103475 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1362.00 EUR
  • 661.20 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103476 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1412.40 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103477 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1412.40 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx103478 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1412.40 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx173833 Abbexa
  • 376.80 EUR
  • 944.40 EUR
  • 493.20 EUR
  • 184.80 EUR
  • 309.60 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody

20-abx129333 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1362.00 EUR
  • 661.20 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Hu01 Cloud-Clone
  • 603.84 EUR
  • 285.60 EUR
  • 1934.40 EUR
  • 724.80 EUR
  • 1329.60 EUR
  • 480.00 EUR
  • 4656.00 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Mu01 Cloud-Clone
  • 605.99 EUR
  • 285.60 EUR
  • 1942.46 EUR
  • 727.49 EUR
  • 1334.98 EUR
  • 481.20 EUR
  • 4676.16 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Mu02 Cloud-Clone
  • 605.99 EUR
  • 285.60 EUR
  • 1942.46 EUR
  • 727.49 EUR
  • 1334.98 EUR
  • 481.20 EUR
  • 4676.16 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Mu03 Cloud-Clone
  • 605.99 EUR
  • 285.60 EUR
  • 1942.46 EUR
  • 727.49 EUR
  • 1334.98 EUR
  • 481.20 EUR
  • 4676.16 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Mu04 Cloud-Clone
  • 605.99 EUR
  • 285.60 EUR
  • 1942.46 EUR
  • 727.49 EUR
  • 1334.98 EUR
  • 481.20 EUR
  • 4676.16 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Ra01 Cloud-Clone
  • 625.34 EUR
  • 290.40 EUR
  • 2015.04 EUR
  • 751.68 EUR
  • 1383.36 EUR
  • 494.40 EUR
  • 4857.60 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Ra02 Cloud-Clone
  • 625.34 EUR
  • 290.40 EUR
  • 2015.04 EUR
  • 751.68 EUR
  • 1383.36 EUR
  • 494.40 EUR
  • 4857.60 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

4-RPA815Ra03 Cloud-Clone
  • 625.34 EUR
  • 290.40 EUR
  • 2015.04 EUR
  • 751.68 EUR
  • 1383.36 EUR
  • 494.40 EUR
  • 4857.60 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068296 Abbexa
  • 878.40 EUR
  • 343.20 EUR
  • 2715.60 EUR
  • 1045.20 EUR
  • 627.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068299 Abbexa
  • 878.40 EUR
  • 343.20 EUR
  • 2715.60 EUR
  • 1045.20 EUR
  • 627.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068302 Abbexa
  • 878.40 EUR
  • 343.20 EUR
  • 2715.60 EUR
  • 1045.20 EUR
  • 627.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068295 Abbexa
  • 844.80 EUR
  • 343.20 EUR
  • 2598.00 EUR
  • 994.80 EUR
  • 594.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068297 Abbexa
  • 844.80 EUR
  • 343.20 EUR
  • 2614.80 EUR
  • 1011.60 EUR
  • 610.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068298 Abbexa
  • 844.80 EUR
  • 343.20 EUR
  • 2614.80 EUR
  • 1011.60 EUR
  • 610.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068300 Abbexa
  • 844.80 EUR
  • 343.20 EUR
  • 2614.80 EUR
  • 1011.60 EUR
  • 610.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein

20-abx068301 Abbexa
  • 844.80 EUR
  • 343.20 EUR
  • 2614.80 EUR
  • 1011.60 EUR
  • 610.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody Pair

20-abx370344 Abbexa
  • 2047.20 EUR
  • 1312.80 EUR
  • 10 × 96 tests
  • 5 × 96 tests

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)

20-abx272743 Abbexa
  • 560.40 EUR
  • 292.80 EUR
  • 1612.80 EUR
  • 760.80 EUR
  • 410.40 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)

20-abx273214 Abbexa
  • 543.60 EUR
  • 292.80 EUR
  • 1545.60 EUR
  • 744.00 EUR
  • 410.40 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)

20-abx274315 Abbexa
  • 510.00 EUR
  • 276.00 EUR
  • 1446.00 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Ra-48T DL Develop 48T 609.6 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Ra-96T DL Develop 96T 793.2 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Ra-48Tests Reddot Biotech 48 Tests 613.2 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Ra-96Tests Reddot Biotech 96 Tests 850.8 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Ra-48Tests Reddot Biotech 48 Tests 640.8 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Ra-96Tests Reddot Biotech 96 Tests 890.4 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Ra-10x96wellstestplate Cloud-Clone 10x96-wells test plate 5552.14 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Ra-1x48wellstestplate Cloud-Clone 1x48-wells test plate 562.42 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Ra-1x96wellstestplate Cloud-Clone 1x96-wells test plate 752.02 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Ra-5x96wellstestplate Cloud-Clone 5x96-wells test plate 3024.07 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

4-SEA815Ra Cloud-Clone
  • 5612.40 EUR
  • 2965.20 EUR
  • 752.40 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Ra-48T Reddot Biotech 48T 465.47 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Ra-96T Reddot Biotech 96T 664.97 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Ra-48T Reddot Biotech 48T 443.3 EUR

Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Ra-96T Reddot Biotech 96T 633.3 EUR

C-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (CAPON) Antibody

abx430457-200ul Abbexa 200 ul 460.8 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit

SCA815Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 6777.36 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit

SCA815Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 663.31 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit

SCA815Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 896.16 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit

SCA815Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 3672.72 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) CLIA Kit

4-SCA815Hu Cloud-Clone
  • 6837.60 EUR
  • 3673.20 EUR
  • 896.40 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Hu-48T DL Develop 48T 574.8 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Hu-96T DL Develop 96T 745.2 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Mu-48T DL Develop 48T 586.8 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

DLR-NOS1-Mu-96T DL Develop 96T 762 EUR

Human Nitric Oxide Synthase 1, Neuronal ELISA Kit (NOS1)

RK01944 Abclonal 96 Tests 625.2 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Hu-48Tests Reddot Biotech 48 Tests 573.6 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Hu-96Tests Reddot Biotech 96 Tests 794.4 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Mu-48Tests Reddot Biotech 48 Tests 586.8 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Mu-96Tests Reddot Biotech 96 Tests 812.4 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Hu-48Tests Reddot Biotech 48 Tests 600 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Hu-96Tests Reddot Biotech 96 Tests 830.4 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Mu-48Tests Reddot Biotech 48 Tests 613.2 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Mu-96Tests Reddot Biotech 96 Tests 850.8 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 5128.02 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 527.48 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 702.12 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

SEA815Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2799.54 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

4-SEA815Hu Cloud-Clone
  • 5188.80 EUR
  • 2739.60 EUR
  • 703.20 EUR
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Hu-48T Reddot Biotech 48T 432.39 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Hu-96T Reddot Biotech 96T 617.72 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Mu-48T Reddot Biotech 48T 443.4 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RDR-NOS1-Mu-96T Reddot Biotech 96T 633.5 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Hu-48T Reddot Biotech 48T 411.8 EUR

Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Hu-96T Reddot Biotech 96T 588.3 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Mu-48T Reddot Biotech 48T 422.3 EUR

Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

RD-NOS1-Mu-96T Reddot Biotech 96T 603.3 EUR

ELISA kit for Rat NOS1 (Nitric Oxide Synthase 1, Neuronal)

ELK1620 ELK Biotech 1 plate of 96 wells 518.4 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody

abx025659-400ul Abbexa 400 ul 627.6 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody

abx025659-80l Abbexa 80 µl 343.2 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody

abx122667-100ug Abbexa 100 ug 469.2 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody

20-abx302365 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ELISA kit for Human NOS1 (Nitric Oxide Synthase 1, Neuronal)

ELK1619 ELK Biotech 1 plate of 96 wells 518.4 EUR

Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Rat)

4-PAA815Ra03 Cloud-Clone
  • 291.60 EUR
  • 2948.40 EUR
  • 735.60 EUR
  • 366.00 EUR
  • 254.40 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Human)

4-PAA815Hu01 Cloud-Clone
  • 278.40 EUR
  • 2742.00 EUR
  • 688.80 EUR
  • 346.80 EUR
  • 249.60 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)

4-PAA815Mu01 Cloud-Clone
  • 283.20 EUR
  • 2805.60 EUR
  • 703.20 EUR
  • 352.80 EUR
  • 250.80 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)

4-PAA815Mu02 Cloud-Clone
  • 283.20 EUR
  • 2805.60 EUR
  • 703.20 EUR
  • 352.80 EUR
  • 250.80 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)

4-PAA815Mu03 Cloud-Clone
  • 283.20 EUR
  • 2805.60 EUR
  • 703.20 EUR
  • 352.80 EUR
  • 250.80 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

ELISA kit for Rat Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein

EK4811 SAB 96 tests 663.6 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (HRP)

20-abx317052 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (FITC)

20-abx317053 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ELISA kit for Human Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein

EK4812 SAB 96 tests 663.6 EUR

Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (Biotin)

20-abx317054 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Infine, abbiamo analizzato l’associazione ad alta affinità del dominio nNOS PDZ con Claudin-3 e Claudin-14, due proteine ​​di membrana tetraspan strettamente collegate che sono componenti essenziali della barriera paracellulare.

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