Approfondimento sulla specificità del legame peptidico al C-terminale del dominio PDZ dell’ossido nitrico sintasi neuronale

Studi precedenti hanno dimostrato che il c-jun e l’ossido nitrico sintasi neurale (nNOS) sono indotti nei motoneuroni danneggiati, ma il loro ruolo nella morte dei motoneuroni rimane poco chiaro.

• Abbiamo ipotizzato che nNOS potrebbe essere un ulteriore effettore della chinasi N-terminale c-jun (JNK) / c-jun nella morte per distacco del motoneurone. Qui abbiamo scoperto che il distacco della radice brachiale ha indotto un aumento transitorio dell’attività di JNK e un aumento di tre e quattro volte rispettivamente di fosfo-c-jun e c-jun; tuttavia, il distacco brachiale ha ridotto l’espressione della proteina nNOS da 4 ore a 14 giorni dopo la lesione.
• A 14 giorni dalla lesione, quasi tutti i motoneuroni nNOS-positivi erano localizzati insieme ai motoneuroni fosfo-c-jun positivi nelle corna addominali omolaterali.
• L’inibitore JNK SP600125, applicato immediatamente dopo la lesione, ha aumentato il livello della proteina nNOS sia nel midollo spinale che nei motoneuroni feriti e ha causato una leggera riduzione della morte dei motoneuroni inibendo la fosforilazione di c-jun 14 giorni dopo la lesione.
• I nostri risultati hanno mostrato che la via di trasduzione del segnale JNK / c-jun è coinvolta nel distacco della radice. L’inibizione della fosforilazione di c-jun impedisce che i livelli di nNOS scendano al di sotto della linea di base nel midollo spinale e mitiga parzialmente la morte del motoneurone dopo l’estrazione della radice.
• Pertanto, l’inibizione della fosforilazione di c-jun o l’aumento del livello della proteina nNOS in un midollo spinale danneggiato in una fase iniziale potrebbero essere utilizzati in futuro come strategie di bersaglio molecolare per prevenire la degenerazione dei motoneuroni durante l’eliminazione delle radici.

 

La coda C-terminale (CT) dell’ossido nitrico sintasi neuronale (nNOS) è un elemento regolatorio che inibisce l’attività di nNOS in assenza di calmodulina legata (CaM).

 

Struttura cristallina del dominio nNOS reduttasi (nNOSr) (Garcin, ED, Bruns, CM, Lloyd, SJ, Hosfield, DJ, Tiso, M., Gachhui, R., Stuehr, DJ, Tainer, JA e Getzoff, ED (2004 ) J Biol. Chem. 279, 37918-37927) ha descritto come la prima metà di CT interagisce con nNOSr e ha quindi fornito un modello per uno studio dettagliato. Abbiamo generato mutanti troncati in nNOS e nNOSr per testare l’importanza di 3 diverse regioni CT.

• L’eliminazione della metà terminale di CT (tutti i residui da Ile1413 a Ser1429), che è invisibile nella struttura cristallina, non ha avuto quasi alcun effetto sul rilascio di NADP +, riduzione della flavina, autoossidazione della flavina, riduzione dell’eme, attività reduttasi o NO attività di sintesi, ma ha impedito un aumento della schermatura FMN che normalmente si verifica in risposta al legame NADPH.
• Un’ulteriore rimozione  dell’alfa-elica CT (residui da 1401 a 1412) ha aumentato significativamente il tasso di rilascio di NADP +, l’autoossidazione della flavina e l’attività della NADPH ossidasi e ha provocato un’esposizione eccessiva del cofattore FMN. Questi effetti sono stati associati con una maggiore attività della reduttasi e una riduzione leggermente ridotta dell’eme e della sintesi di NO.
• L’ulteriore rimozione dei residui dopo Gly1396 (troncamento CT completo) ha migliorato gli effetti sopra menzionati e ha ulteriormente modificato l’interazione di NADP + con FAD, attenuando la soppressione cinetica della riduzione della flavina e ulteriormente ridotta la riduzione dell’eme e la sintesi di NO.
•  I nostri risultati mostrano che la TC esercita effetti sia sfaccettati che regiospecifici sull’attività catalitica e sui comportamenti correlati, fornendo così nuove informazioni sui meccanismi che regolano la catalisi di nNOS.

I domini PDZ sono motivi di interazione proteica multifunzionale che spesso si legano al C-terminale delle proteine ​​​​bersaglio.

 L’ossido nitrico (NO), una molecola di segnalazione endogena, svolge un ruolo chiave nel funzionamento del sistema nervoso, immunitario e cardiovascolare. Sebbene ci siano molte funzioni fisiologiche per l’NO neuronale prodotto principalmente dalla NO sintasi neuronale (nNOS), l’eccessiva attività di nNOS media il danno cerebrale nell’ischemia cerebrale e nei modelli animali del morbo di Parkinson.

Pertanto, la localizzazione subcellulare dell’attività di nNOS deve essere strettamente regolata. Per determinare i ligandi del dominio nNOS PDZ, abbiamo cercato 13 miliardi di peptidi diversi e abbiamo scoperto che il dominio nNOS-PDZ si lega strettamente ai peptidi che terminano in Asp-X-Val. Questo differisce dall’unico consenso noto (Thr / Ser) -X-Val che interagisce con i domini PDZ di PSD-95. La preferenza per Asp in posizione -2 è mediata da Tyr-77 di nNOS. La sostituzione di Y77D78 con H77E78 cambia la specificità del legame da Asp-X-Val a Thr-X-Val. A seguito del consenso di Asp-X-Val, sono state identificate le proteine ​​candidate che interagiscono con nNOS, inclusi i recettori del glutammato e della melatonina. I nostri risultati mostrano che i domini PDZ hanno una chiara specificità di legame peptidico.

 

L’ossido nitrico sintasi neurale, a differenza delle sue controparti endoteliali e inducibili, mostra un dominio PDZ (PSD-95 / Dlg / ZO-1) situato al suo N-terminale coinvolto nel targeting subcellulare.

 Il C-terminale delle varie proteine ​​cellulari si inserisce nel solco di legame di questo dominio PDZ e definisce la distribuzione subcellulare della NOS neuronale (nNOS). I meccanismi molecolari alla base di queste interazioni sono poco conosciuti poiché il dominio nNOS PDZ può sembrare mostrare specificità di legame di classe I, classe II e classe III.

Inoltre  , è stato recentemente suggerito che il dominio nNOS PDZ si leghi con un’affinità molto bassa al C-terminale delle proteine ​​bersaglio e che la necessaria interazione laterale concomitante debba avvenire affinché avvenga il legame. Descriviamo qui che un dominio nNOS PDZ può comportarsi come un dominio PDZ di classe III in buona fede e legarsi a sequenze C-terminali con residui acidi nella posizione P-2 con costanti micromolari basse. Può verificarsi anche il legame alle sequenze C-terminali con un residuo idrofobo in P-2 e un residuo acido in P-3 (classe II), sebbene interazioni che coinvolgono residui che si estendono a P-7 mediano questo tipo di legame.

Questo comportamento promiscuo si estende anche alla sua associazione con sequenze di classe I, che devono mostrare un residuo di Glu in P-3 e un residuo di Thr in P-2. Mediante mutagenesi sito-diretta e spettroscopia NMR, siamo stati in grado di identificare i residui coinvolti in ogni tipo specifico di legame e razionalizzare i meccanismi utilizzati per riconoscere i partner vincolanti.

 

Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E03C2363-192T	BlueGene	192 tests	1524 EUR
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E03C2363-48	BlueGene	1 plate of 48 wells	624 EUR
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E03C2363-96	BlueGene	1 plate of 96 wells	822 EUR
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E01C2363-192T	BlueGene	192 tests	1524 EUR
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E01C2363-48	BlueGene	1 plate of 48 wells	624 EUR
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E01C2363-96	BlueGene	1 plate of 96 wells	822 EUR
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E09C2363-192T	BlueGene	192 tests	1524 EUR
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E09C2363-48	BlueGene	1 plate of 48 wells	624 EUR
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E09C2363-96	BlueGene	1 plate of 96 wells	822 EUR
nNOS (Neuronal Nitric Oxide Synthase)
RA19011	Neuromics	100 uL	451.2 EUR
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E05C2363-192T	BlueGene	192 tests	1524 EUR
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E05C2363-48	BlueGene	1 plate of 48 wells	624 EUR
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit
E05C2363-96	BlueGene	1 plate of 96 wells	822 EUR
Neuronal Nitric Oxide Synthase (Nnos) Antibody
20-abx114082	Abbexa	878.40 EUR 477.60 EUR	150 ul 50 ul
nNOS-C-terminus (Neuronal Nitric Oxide Synthase)
P24287	Neuromics	50 ug Blocking Peptide	208.8 EUR
nNOS-C-terminus (Neuronal Nitric Oxide Synthase)
RA24287	Neuromics	100 uL	646.8 EUR
Rat neuronal nitric oxide synthase,nNOS ELISA Kit
YLA0034RA-48T	Shanghai YL Biotech	48T	465 EUR
Rat neuronal nitric oxide synthase,nNOS ELISA Kit
YLA0034RA-96T	Shanghai YL Biotech	96T	600 EUR
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit
1-CSB-E13872h	Cusabio	843.60 EUR 5811.60 EUR 3084.00 EUR	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit
CSB-E13872h-24T	Cusabio	1 plate of 24 wells	198 EUR
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit
GA-E0550RT-48T	GenAsia Biotech	48T	380.4 EUR
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit
GA-E0550RT-96T	GenAsia Biotech	96T	595.2 EUR
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit
QY-E11102	Qayee Biotechnology	96T	433.2 EUR
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit
CSB-E14034r-24T	Cusabio	1 plate of 24 wells	198 EUR
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit
1-CSB-E14034r	Cusabio	843.60 EUR 5811.60 EUR 3084.00 EUR	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Mouse neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit
QY-E21072	Qayee Biotechnology	96T	433.2 EUR
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit
CSB-E13273m-24T	Cusabio	1 plate of 24 wells	198 EUR
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit
1-CSB-E13273m	Cusabio	843.60 EUR 5811.60 EUR 3084.00 EUR	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody
20-abx329607	Abbexa	376.80 EUR 292.80 EUR	100 ug 50 ug
Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit
YLA0003CH-48T	Shanghai YL Biotech	48T	540 EUR
Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit
YLA0003CH-96T	Shanghai YL Biotech	96T	645 EUR
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)
KTE100825-48T	Abbkine	48T	424.8 EUR
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)
KTE100825-5platesof96wells	Abbkine	5 plates of 96 wells	2702.4 EUR
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1)
KTE100825-96T	Abbkine	96T	686.4 EUR
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx595427-96tests	Abbexa	96 tests	764.4 EUR
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (HRP)
20-abx314989	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit
abx195276-96tests	Abbexa	96 tests	990 EUR
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit
20-abx492066	Abbexa	10282.80 EUR 5472.00 EUR 1262.40 EUR	10 × 96 tests 5 × 96 tests 96 tests
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (FITC)
20-abx314990	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx256109-96tests	Abbexa	96 tests	801.6 EUR
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
20-abx155900	Abbexa	8684.40 EUR 4626.00 EUR 1074.00 EUR	10 × 96 tests 5 × 96 tests 96 tests
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx055223-96tests	Abbexa	96 tests	801.6 EUR
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx570103-96tests	Abbexa	96 tests	801.6 EUR
Pig Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx361104-96tests	Abbexa	96 tests	990 EUR
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit
ER0144	FN Test	96T	571.5 EUR
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit
abx195274-96tests	Abbexa	96 tests	990 EUR
Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit
abx195275-96tests	Abbexa	96 tests	990 EUR
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (Biotin)
20-abx314991	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
20-abx152535	Abbexa	7970.40 EUR 4250.40 EUR 990.00 EUR	10 × 96 tests 5 × 96 tests 96 tests
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx253515-96tests	Abbexa	96 tests	801.6 EUR
Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx254292-96tests	Abbexa	96 tests	801.6 EUR
Sheep Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx364404-96tests	Abbexa	96 tests	1111.2 EUR
Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx055268-96tests	Abbexa	96 tests	801.6 EUR
Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx513552-96tests	Abbexa	96 tests	801.6 EUR
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx570102-96tests	Abbexa	96 tests	801.6 EUR
Mouse NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit
EM1235	FN Test	96T	571.5 EUR
Rabbit Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx362730-96tests	Abbexa	96 tests	990 EUR
Monkey Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx358861-96tests	Abbexa	96 tests	990 EUR
CLIA kit for Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-CL-R0746	Elabscience Biotech	1 plate of 96 wells	700.8 EUR
Chicken Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx356455-96tests	Abbexa	96 tests	990 EUR
ELISA kit for Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-EL-R1438	Elabscience Biotech	1 plate of 96 wells	640.8 EUR
CLIA kit for Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-CL-M0697	Elabscience Biotech	1 plate of 96 wells	700.8 EUR
CLIA kit for Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-CL-H0537	Elabscience Biotech	1 plate of 96 wells	700.8 EUR
ELISA kit for Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-EL-M1281	Elabscience Biotech	1 plate of 96 wells	640.8 EUR
ELISA kit for Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
E-EL-H0742	Elabscience Biotech	1 plate of 96 wells	640.8 EUR
NOS1/nNOS ELISA Kit| Mouse Nitric Oxide Synthase 1, Neuronal ELI
EF013784	Lifescience Market	96 Tests	826.8 EUR
NOS1/nNOS ELISA Kit| Rat Nitric Oxide Synthase 1, Neuronal ELISA
EF017083	Lifescience Market	96 Tests	826.8 EUR
Guinea pig Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit
abx357551-96tests	Abbexa	96 tests	990 EUR
Rat Neuronal Nitric Oxide Synthase ELISA kit
E02N0069-192T	BlueGene	192 tests	1524 EUR
Rat Neuronal Nitric Oxide Synthase ELISA kit
E02N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Rat Neuronal Nitric Oxide Synthase ELISA kit
E02N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Pig Neuronal Nitric Oxide Synthase ELISA kit
E07N0069-192T	BlueGene	192 tests	1524 EUR
Pig Neuronal Nitric Oxide Synthase ELISA kit
E07N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Pig Neuronal Nitric Oxide Synthase ELISA kit
E07N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Dog Neuronal Nitric Oxide Synthase ELISA kit
E08N0069-192T	BlueGene	192 tests	1524 EUR
Dog Neuronal Nitric Oxide Synthase ELISA kit
E08N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Dog Neuronal Nitric Oxide Synthase ELISA kit
E08N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Goat Neuronal Nitric Oxide Synthase ELISA kit
E06N0069-192T	BlueGene	192 tests	1524 EUR
Goat Neuronal Nitric Oxide Synthase ELISA kit
E06N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Goat Neuronal Nitric Oxide Synthase ELISA kit
E06N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Mouse Neuronal Nitric Oxide Synthase ELISA kit
E03N0069-192T	BlueGene	192 tests	1524 EUR
Mouse Neuronal Nitric Oxide Synthase ELISA kit
E03N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Mouse Neuronal Nitric Oxide Synthase ELISA kit
E03N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Human Neuronal Nitric Oxide Synthase ELISA kit
E01N0069-192T	BlueGene	192 tests	1524 EUR
Human Neuronal Nitric Oxide Synthase ELISA kit
E01N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Human Neuronal Nitric Oxide Synthase ELISA kit
E01N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Rabbit Neuronal Nitric Oxide Synthase ELISA kit
E04N0069-192T	BlueGene	192 tests	1524 EUR
Rabbit Neuronal Nitric Oxide Synthase ELISA kit
E04N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Rabbit Neuronal Nitric Oxide Synthase ELISA kit
E04N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Monkey Neuronal Nitric Oxide Synthase ELISA kit
E09N0069-192T	BlueGene	192 tests	1524 EUR
Monkey Neuronal Nitric Oxide Synthase ELISA kit
E09N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Monkey Neuronal Nitric Oxide Synthase ELISA kit
E09N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Guinea pig Neuronal Nitric Oxide Synthase ELISA kit
E05N0069-192T	BlueGene	192 tests	1524 EUR
Guinea pig Neuronal Nitric Oxide Synthase ELISA kit
E05N0069-48	BlueGene	1 plate of 48 wells	624 EUR
Guinea pig Neuronal Nitric Oxide Synthase ELISA kit
E05N0069-96	BlueGene	1 plate of 96 wells	822 EUR
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103472	Abbexa	477.60 EUR 159.60 EUR 1328.40 EUR 644.40 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103473	Abbexa	493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103474	Abbexa	493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103475	Abbexa	493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103476	Abbexa	510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103477	Abbexa	510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx103478	Abbexa	510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx173833	Abbexa	376.80 EUR 944.40 EUR 493.20 EUR 184.80 EUR 309.60 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody
20-abx129333	Abbexa	493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Hu01	Cloud-Clone	603.84 EUR 285.60 EUR 1934.40 EUR 724.80 EUR 1329.60 EUR 480.00 EUR 4656.00 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Mu01	Cloud-Clone	605.99 EUR 285.60 EUR 1942.46 EUR 727.49 EUR 1334.98 EUR 481.20 EUR 4676.16 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Mu02	Cloud-Clone	605.99 EUR 285.60 EUR 1942.46 EUR 727.49 EUR 1334.98 EUR 481.20 EUR 4676.16 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Mu03	Cloud-Clone	605.99 EUR 285.60 EUR 1942.46 EUR 727.49 EUR 1334.98 EUR 481.20 EUR 4676.16 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Mu04	Cloud-Clone	605.99 EUR 285.60 EUR 1942.46 EUR 727.49 EUR 1334.98 EUR 481.20 EUR 4676.16 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Ra01	Cloud-Clone	625.34 EUR 290.40 EUR 2015.04 EUR 751.68 EUR 1383.36 EUR 494.40 EUR 4857.60 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Ra02	Cloud-Clone	625.34 EUR 290.40 EUR 2015.04 EUR 751.68 EUR 1383.36 EUR 494.40 EUR 4857.60 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
4-RPA815Ra03	Cloud-Clone	625.34 EUR 290.40 EUR 2015.04 EUR 751.68 EUR 1383.36 EUR 494.40 EUR 4857.60 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068296	Abbexa	878.40 EUR 343.20 EUR 2715.60 EUR 1045.20 EUR 627.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068299	Abbexa	878.40 EUR 343.20 EUR 2715.60 EUR 1045.20 EUR 627.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068302	Abbexa	878.40 EUR 343.20 EUR 2715.60 EUR 1045.20 EUR 627.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Human Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068295	Abbexa	844.80 EUR 343.20 EUR 2598.00 EUR 994.80 EUR 594.00 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068297	Abbexa	844.80 EUR 343.20 EUR 2614.80 EUR 1011.60 EUR 610.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068298	Abbexa	844.80 EUR 343.20 EUR 2614.80 EUR 1011.60 EUR 610.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068300	Abbexa	844.80 EUR 343.20 EUR 2614.80 EUR 1011.60 EUR 610.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Protein
20-abx068301	Abbexa	844.80 EUR 343.20 EUR 2614.80 EUR 1011.60 EUR 610.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody Pair
20-abx370344	Abbexa	2047.20 EUR 1312.80 EUR	10 × 96 tests 5 × 96 tests
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)
20-abx272743	Abbexa	560.40 EUR 292.80 EUR 1612.80 EUR 760.80 EUR 410.40 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)
20-abx273214	Abbexa	543.60 EUR 292.80 EUR 1545.60 EUR 744.00 EUR 410.40 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Nitric Oxide Synthase 1, Neuronal (NOS1) Antibody (Biotin)
20-abx274315	Abbexa	510.00 EUR 276.00 EUR 1446.00 EUR 693.60 EUR 393.60 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Ra-48T	DL Develop	48T	609.6 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Ra-96T	DL Develop	96T	793.2 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Ra-48Tests	Reddot Biotech	48 Tests	613.2 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Ra-96Tests	Reddot Biotech	96 Tests	850.8 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Ra-48Tests	Reddot Biotech	48 Tests	640.8 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Ra-96Tests	Reddot Biotech	96 Tests	890.4 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Ra-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	5552.14 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Ra-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	562.42 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Ra-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	752.02 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Ra-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	3024.07 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
4-SEA815Ra	Cloud-Clone	5612.40 EUR 2965.20 EUR 752.40 EUR	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Ra-48T	Reddot Biotech	48T	465.47 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Ra-96T	Reddot Biotech	96T	664.97 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Ra-48T	Reddot Biotech	48T	443.3 EUR
Rat Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Ra-96T	Reddot Biotech	96T	633.3 EUR
C-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (CAPON) Antibody
abx430457-200ul	Abbexa	200 ul	460.8 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit
SCA815Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	6777.36 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit
SCA815Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	663.31 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit
SCA815Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	896.16 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1)CLIA Kit
SCA815Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	3672.72 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) CLIA Kit
4-SCA815Hu	Cloud-Clone	6837.60 EUR 3673.20 EUR 896.40 EUR	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Hu-48T	DL Develop	48T	574.8 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Hu-96T	DL Develop	96T	745.2 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Mu-48T	DL Develop	48T	586.8 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
DLR-NOS1-Mu-96T	DL Develop	96T	762 EUR
Human Nitric Oxide Synthase 1, Neuronal ELISA Kit (NOS1)
RK01944	Abclonal	96 Tests	625.2 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Hu-48Tests	Reddot Biotech	48 Tests	573.6 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Hu-96Tests	Reddot Biotech	96 Tests	794.4 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Mu-48Tests	Reddot Biotech	48 Tests	586.8 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Mu-96Tests	Reddot Biotech	96 Tests	812.4 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Hu-48Tests	Reddot Biotech	48 Tests	600 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Hu-96Tests	Reddot Biotech	96 Tests	830.4 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Mu-48Tests	Reddot Biotech	48 Tests	613.2 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Mu-96Tests	Reddot Biotech	96 Tests	850.8 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	5128.02 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	527.48 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	702.12 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
SEA815Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	2799.54 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
4-SEA815Hu	Cloud-Clone	5188.80 EUR 2739.60 EUR 703.20 EUR	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Hu-48T	Reddot Biotech	48T	432.39 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Hu-96T	Reddot Biotech	96T	617.72 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Mu-48T	Reddot Biotech	48T	443.4 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RDR-NOS1-Mu-96T	Reddot Biotech	96T	633.5 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Hu-48T	Reddot Biotech	48T	411.8 EUR
Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Hu-96T	Reddot Biotech	96T	588.3 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Mu-48T	Reddot Biotech	48T	422.3 EUR
Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit
RD-NOS1-Mu-96T	Reddot Biotech	96T	603.3 EUR
ELISA kit for Rat NOS1 (Nitric Oxide Synthase 1, Neuronal)
ELK1620	ELK Biotech	1 plate of 96 wells	518.4 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody
abx025659-400ul	Abbexa	400 ul	627.6 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody
abx025659-80l	Abbexa	80 µl	343.2 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody
abx122667-100ug	Abbexa	100 ug	469.2 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody
20-abx302365	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
ELISA kit for Human NOS1 (Nitric Oxide Synthase 1, Neuronal)
ELK1619	ELK Biotech	1 plate of 96 wells	518.4 EUR
Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Rat)
4-PAA815Ra03	Cloud-Clone	291.60 EUR 2948.40 EUR 735.60 EUR 366.00 EUR 254.40 EUR	100ul 10ml 1ml 200ul 20ul
Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Human)
4-PAA815Hu01	Cloud-Clone	278.40 EUR 2742.00 EUR 688.80 EUR 346.80 EUR 249.60 EUR	100ul 10ml 1ml 200ul 20ul
Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)
4-PAA815Mu01	Cloud-Clone	283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80 EUR	100ul 10ml 1ml 200ul 20ul
Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)
4-PAA815Mu02	Cloud-Clone	283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80 EUR	100ul 10ml 1ml 200ul 20ul
Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody (Mouse)
4-PAA815Mu03	Cloud-Clone	283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80 EUR	100ul 10ml 1ml 200ul 20ul
ELISA kit for Rat Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
EK4811	SAB	96 tests	663.6 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (HRP)
20-abx317052	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (FITC)
20-abx317053	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
ELISA kit for Human Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
EK4812	SAB	96 tests	663.6 EUR
Carboxyl-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase Protein (NOS1AP) Antibody (Biotin)
20-abx317054	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug

Infine, abbiamo analizzato l’associazione ad alta affinità del dominio nNOS PDZ con Claudin-3 e Claudin-14, due proteine ​​di membrana tetraspan strettamente collegate che sono componenti essenziali della barriera paracellulare.