Rilevamento diretto del coronavirus felino con tre test immunocromatografici rapidi basati sull’antigene e PCR in tempo reale nei rifugi per gatti

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Effetto dell’inattivazione termica sulla rilevazione della sindrome respiratoria acuta grave – coronavirus-2 (SARS-CoV-2) con   reazione a catena della polimerasi a trascrizione inversa in   tempo reale (rRT-    PCR   ): evidenza da uno studio in Etiopia

La malattia da coronavirus 2019 (COVID-19) è di grande importanza per la salute pubblica e il suo campione dovrebbe essere maneggiato in sicurezza a causa delle preoccupazioni sulla possibile trasmissione agli operatori sanitari. L’inattivazione termica del campione prima dell’isolamento dell’acido nucleico può consentire processi di analisi sicuri. Pertanto, è importante valutare l’effetto dell’inattivazione del calore sul rilevamento di SARS-CoV-2 RT-PCR in condizioni di risorse limitate.
Uno studio sperimentale è stato condotto presso l’Ethiopian Public Health Institute (EPHI) dal 25 settembre al 15 ottobre 2020. Sono stati raccolti un totale di 188 tamponi orofaringei da casi sospetti di COVID-19 riferiti a EPHI per il test SARS COV-2. 
Un lotto del campione è stato inattivato a 56°C per 30 minuti e l’altro   lotto è stato conservato a 4°C per un periodo di tempo simile. L’estrazione e il rilevamento dell’RNA sono stati eseguiti utilizzando i protocolli del kit DAAN Gene   . Abbott m2000 RT-PCR è stato utilizzato per l’amplificazione e il rilevamento. L’analisi dei dati è stata eseguita utilizzando SPSS versione 23.0; I test di correlazione del chi quadrato e di Pearson sono stati utilizzati per l’analisi qualitativa e semiquantitativa. Un valore p <0,05 è stato considerato statisticamente significativo.
Di tutti i 188 campioni, 119 (63,3%) erano positivi e 69 (36,7%) negativi nel gruppo non inattivato. Al contrario, 115 (61,2%) campioni erano positivi e 73 (38,8) negativi nel lotto di campioni inattivati ​​al calore. L’indice di positività tra i gruppi non ha mostrato differenze statisticamente significative (p> 0,05). La differenza media nel valore di soglia del ciclo (Ct) tra i due   gruppi del gene ORF1a / b e del gene N era 0,042 (IC 95% – 0,247-0,331; t = 0,28; p = 0,774) e 0,38 (IC 95% 0,097-0,682; t = 2,638; p = 0,010).
L’inattivazione termica a 56°C per 30 min non ha influito sulla rilevazione qualitativa di SARS-CoV-2 rRT-PCR. Tuttavia, la scoperta ha mostrato che c’era un aumento statisticamente significativo del valore Ct dopo l’inattivazione termica rispetto ai campioni non trattati. Pertanto, i risultati falsi negativi per campioni con un valore Ct elevato (Ct> 35) si sono rivelati una sfida per questo protocollo. Pertanto, dovrebbero essere studiati metodi alternativi di inattivazione e considerare ulteriori ricerche.

DirectDetect SARS-CoV-2 Direct RT –   PCR in   tempo  reale    utilizzando i campioni dei pazienti

Il COVID-19 è una malattia infettiva che ha causato una pandemia globale che colpisce persone in tutto il mondo. Poiché il rilevamento delle malattie e l’introduzione dei vaccini continuano, sono ancora necessari strumenti diagnostici efficaci per soddisfare le esigenze di test continui. Questo studio preliminare ha valutato un nuovo test diagnostico SARS-CoV-2 chiamato DirectDetect SARS-CoV-2 Real Time Direct Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) basato su una   dimensione del campione limitata di 24 campioni di respiro con 14 SARS-CoV – 2 -pazienti positivi. Il test è superiore ad altri test disponibili in commercio in quanto non richiede il mezzo di trasporto del virus o l’estrazione dell’RNA virale prima dell’amplificazione e del rilevamento dell’acido nucleico.
  • Questa capacità trasforma ore di tempo di preparazione del campione in una procedura che richiede minuti, eliminando la necessità di molti reagenti costosi che possono essere difficili da ottenere durante il picco nel test dell’acido nucleico durante una pandemia.
  • I risultati mostrano una concordanza positiva del   94,7, 100 e 94,7% tra tamponi asciutti con campioni, campioni trattati e non trattati testati con DirectDetect SARS-CoV-2   RT-PCR diretta in tempo reale rispetto ai test utilizzati in laboratorio clinico, rispettivamente.
  • I risultati indicano che DirectDetect può essere utilizzato per molti diversi tipi di campioni riducendo il numero di reagenti e il tempo necessario per la diagnosi.
  • Sebbene questo studio mostri risultati promettenti utilizzando i risultati DirectDetect, è necessaria un’ulteriore convalida di questo test con un set più ampio di campioni per valutare le prestazioni effettive di questo test.
  • Il rilevamento tempestivo dei fattori eziologici è importante per il successo del trattamento di un ascesso ileopsoas (IPA). Lo scopo di questo studio era di testare l’utilità clinica della reazione a catena della polimerasi (PCR) in tempo reale che prende di mira il   gene mecA  per gli stafilococchi meticillino-resistenti (MRS) e il gene 16S rRNA per i pan-batteri. Il nostro studio diagnostico retrospettivo ha incluso 22 pazienti che mostravano IPA e quattro pazienti con aree non infettive del tumore ileopsoas sottoposti a tomografia computerizzata   o biopsia ecografica e/o chirurgia.
I sintomi clinici, i dati sierici, l’analisi di imaging e la coltura microbiologica dei tessuti sono stati utilizzati per diagnosticare l’IPA. L’accuratezza diagnostica della PCR in tempo reale è stata determinata sulla base della diagnosi dei risultati dell’IPA e della coltura microbica. La coltura microbica è risultata positiva per 12 casi di IPA, di cui 2 infezioni da MRSA. Dei 12 IPA positivi in ​​coltura, 16S rRNA-PCR era positivo in 12 e MRS-PCR in due. Dei 10 casi di IPA negativi alla coltura, inclusi 3 casi di tubercolosi, 16S   rRNA-PCR era positivo in 8 e MRS-PCR era positivo in 2. Nei pazienti con massa ileopsoas non infettiva, né 16S rRNA né MRS-PCR hanno rilevato un’infezione batterica . GOTTA.
Sensibilità, specificità, valori predittivi positivi e negativi di 16S rRNA-PCR nella diagnosi di IPA erano rispettivamente 0,91, 1,00, 1,00 e 0,67, mentre quelli per la diagnosi di infezione da MRS mediante MRS-PCR erano 1,00, 0,92, 1,00 , e 0,50 rispettivamente. La PCR in tempo reale mirata al DNA batterico può rilevare il DNA batterico nei casi negativi alla coltura e fornire una migliore rilevabilità dell’infezione da MRS nei pazienti IPA.

Test PCR  quantitativi   in ​​tempo  reale   per     la diagnosi simultanea del virus della laringotracheite infettiva, del virus della malattia di Newcastle e del metapneumovirus aviario nel pollame

La malattia di Newcastle (ND), la laringotracheite infettiva (ILT) e il metapneumovirus aviario (aMPV) possono essere tutti simili, il che rende fondamentale una rapida differenziazione. Qui abbiamo adattato i test diagnostici molecolari preesistenti per NDV e ILTV e sviluppato nuovi test per aMPV A e B da utilizzare in   condizioni di termociclaggio sincronizzato. Tutti i test sono stati eseguiti in modo equivalente con linearità nell’intervallo dinamico di 5 log   10   , limite di rilevamento ripetibile (R²> 0,99) di ≥ 10 copie target ed efficienze di amplificazione comprese tra 86,8% e 98,2%. L’uso di campioni biologici per NDV e ILTV ha mostrato una specificità del 100%. Condizioni di amplificazione identiche semplificheranno le procedure di rilevamento nei laboratori diagnostici.
Lo scopo di questo studio era il rilevamento diretto del coronavirus felino mediante PCR in tempo reale e tre diversi test di immunocromatografia rapida (RIM) per rilevare gli antigeni nei campioni felini dei rifugi. L’utilità dei test RIM è stata confrontata in base alla sensibilità e specificità calcolate per ciascuno dei test RIM. Sono stati testati  settanta campioni fecali di gatti di rifugio in quarantena   . Dei 70 campioni analizzati mediante PCR in tempo reale, 44 (62,9%) erano positivi.

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
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hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
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hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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  • 50 rxns

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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  • 50 rxns

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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  • 50 rxns

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
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hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
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hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
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  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097627 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097628 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097631 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097632 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097633 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097634 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097636 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097637 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097638 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097639 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097640 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097642 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097643 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097644 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097645 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097647 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097648 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097650 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097651 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097652 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097653 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097654 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097657 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097659 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097706 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097708 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097709 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097711 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097712 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097714 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097715 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097716 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097717 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097719 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097720 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097721 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097722 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097723 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097724 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097725 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097726 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097728 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-190b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097729 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-192 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097731 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-193a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097732 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-193b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097733 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-194 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097734 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-195 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097735 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-197 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097738 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-198 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097739 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-199a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097740 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-199b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097741 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-200a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097742 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-200b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097743 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-202 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097745 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-203 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097746 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-204 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097747 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-205 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097748 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-206 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097749 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-208a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097750 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-208b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097751 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-211 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097753 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-212 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097754 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-214 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097755 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-215 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097756 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-217 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097757 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-218 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097758 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-219 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097759 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-223 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097764 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-224 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097765 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-298 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097768 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-299 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097769 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-320a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097774 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-320b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097775 Abbexa
  • 878.40 EUR
  • 1245.60 EUR
  • 627.60 EUR
  • 100 rxns
  • 200 rxns
  • 50 rxns
Significativamente più gatti (   p    <0,05) sono risultati positivi che negativi. Né l’età né il sesso dei gatti hanno giocato un ruolo significativo (   p    > 0,05) nell’escrezione virale. La sensibilità dei test RIM è risultata bassa (<35%; test RIM A e C) a un livello soddisfacente (> 50%, test RIM B). Il numero di particelle virali determinato dall’analisi RT-PCR in tempo reale non era significativamente correlato ai risultati rilevati in nessuno dei saggi RIM (   p    > 0,05). I risultati di questo studio indicano che l’uso dei test antigenici rapidi RIM nello screening di routine per lo stato di eliminazione di FCoV nei gatti da rifugio è limitato a causa dell’elevato numero di risultati falsi negativi.

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